Proteomics

Proteomics

Protein profiling resources

The Oxford Genomics Centre is committed to expanding its portfolio of supported technologies for biomarkers discovery.

To this end, we have been the first in UK to implement and offer the newest version of the Proximity Extension Assay (PEA) technology by Olink. With Explore, a high-throughput protein profiling, PEA is coupled with NGS to vastly increase the number of detectable proteins from small volumes of samples.

We offer support from study design to delivery of high quality data in the form of normalised protein counts for any combination of the available panels available from Olink.

 

Olink Explore

Olink

Olink’s PEA technology gains exceptional specificity from the use of dual antibodies recognition. Only when two specific antibodies bind the target protein, the oligo tag each enzyme is conjugated to can be amplified. This strategy prevents unspecific antibody recognition to cause false positive signals.

At OGC we sequence library generated with the Olink Explore workflow on our Illumina Novaseq 6000 to provide the most cost effective and time efficient sequencing results.

All datasets generated with Olink Explore are rigorously Quality Controlled before dissemination to our customers.

 

Guidelines:

  • Please provide each sample in temperature-resistant, non-protein binding PCR plate(s). Maximum 100ul. The volume required per sample ranges from 40ul to 100ul depending on the number of panels requested. We can arrange for any unused material to be shipped back.
  • Use require samples to be delivered in PCR-clean 96-well PCR-plates, with full skirt (e.g. ThermoFisher Scientific #AB0800 with MicroAmp seal #4306311 from ThermoFisher Scientific). All need to be -80°C, dry-ice resistant and easily resealable.
  • Ensure that your samples are randomised and compatible with our plate layout, (column 12 must be empty). We can advise on how to randomise your samples.
  • Ensure that each well is separately sealed using an adhesive film or individual seals.Mark the Box(es) with the quote number. Clearly mark sample plates with the quote number and, if more than one plate, a simple alphanumeric code for each plate.
  • Use anonymised sample identification names/numbers in the sample submission form. No duplicate names are allowed. For duplicate samples/names, add an extra discriminator (i.e. Sample A_1 and Sample A_2).
  • Plates; use the same plate ID in the sample submission form as used to label on the plate.
    Note: Empty wells scattered within the plates will be charged as samples.

Plate layout

  • Leave Al2-H12 (12th column) empty for controls supplied by Olink. Start in A1 and fill samples column wise.

Shipping

  • Samples should be shipped on dry ice.
  • Make sure the plates are securely sealed, add extra adsorption paper in case of leakage and make sure to place the samples in a sealed bag.
  • Ensure that the heavy dry ice will not damage the samples during shipment.
  • Place a document referencing the Quote number identifying the samples and the project they belong to.
  • Upon receipt, samples will be stored at -80°C.