Sequencing technology at the cutting edge

PolyA – libraries are prepared from polyadenylated (mature mRNA) transcripts.

Ribosomal/ globin depletion – libraries are prepared from RNA depleted for highly abundant sequences.

3’mRNA – this prep generates short tags close to the 3? end of the transcripts so that simple gene counts can be performed.

Ultra Low RNA – we can also generate libraries from sorted single cells or single-cell amounts of RNA.

Read more about our PolyA, ribosomal depletion, 3’mRNA and ultra low approaches.

No isolation – enrichment for poly(A)+ sequences or depletion of ribosomal RNA are not suitable for all species and situations. We can also generate libraries without first performing either of these steps.

Virus RNA – we specialise in methods for whole-genome sequencing of clinically important viruses, including HCV and HIV from low-input, low-copy samples such as plasma. Please contact us to discuss your specific needs.

TCR – we can perform a targeted enrichment to allow you to study only the T cell repertoire of your bulk samples.

Small RNA – the discovery and profiling of microRNAs and other small non-coding RNA from any organism, using specialised library preparation methods.

Read more about our small RNA approach.

In addition to our ultra low library preparation capability, we also have a dedicated single cell platform that supports a number of applications: 10X Genomics Chromium.

Single Cell Gene Expression – The Chromium Single Cell 3’ Solution provides high-throughput, single cell expression measurements that enable discovery of gene expression dynamics and molecular profiling of individual cell types. Cell surface protein information can be added with the use of Totalseq Antibodies.

Single Cell Immune Profiling – The Chromium Single Cell V(D)J Solution enables profiling of full-length paired V(D)J transcripts from thousands of lymphocytes.  This solution allows assembly of full-length V(D)J sequences on a cell-by-cell basis, providing high-resolution insights into the adaptive immune system. Gene expression can be combined with immunophenotyping using the Totalseq Antibodies to better describe immune cell subsets and functional states.

Single Cell ATAC – The Assay for Transposase Accessible Chromatin (ATAC) for epigenomic analysis of thousands of individual nuclei provides insight into cell types and cell states for deeper understanding of gene regulatory mechanisms.

Single Cell Multiome ATAC and Gene Expression – Designed to simultaneously profile gene expression and open chromatin from the same cell allowing characterisation of cell types and states, and uncover gene regulatory programs.

Targeted Gene Expression – A defined set of transcripts from single cells or tissue sections can be profiled with Targeted Gene Expression. Customizable, comprehensive gene panels will allow reduced sequencing costs of your samples.

Available panels include Human Pan-Cancer Panel, Human Immunology Panel, Human Gene Signature Panel and the Human Neuroscience Panel. It is also possible to design custom panels and further information can be found on the 10X site.

Visium Spatial Gene Expression – This technology allows mapping of the whole transcriptome with morphological context of the tissue. Oxford Genomic Centre can support library preparation and sequencing of cDNA samples derived from Visium solution.

Feature Barcode Technology –
• Antibodies for cell surface protein analysis can be added for an extra layer of information. This solution is compatible with 3 prime and V(D)J Solutions. Individual antibodies or pre-designed panels are available from Biolegend.
• 10X’s CellPlex solution for cell hashing & multiplexing uses a series of oligonucleotides conjugated to a lipid, allowing for subsequent pooling of multiple samples to be run on single channel. Benefits of hashing include a reduction of doublet rate, minimising of batch effects and a cost reduction of complex experiment
• Peptide-MHC multimers to measure antigen specificity. Assess the specificity of the adaptive immune system at single cell resolution with simultaneous capture of paired T cell receptors and peptide-MHC multimers, or immunoglobulin heavy and light chains and their cognate antigens.

Can’t see the library type you’re looking for? OGC also supports its users by QC’ing and sequencing their premade, pooled libraries, allowing you much greater flexibility to push the boundaries with your experiment.