Single-Cells

The Chromium Single Cell 3’ Solution provides high-throughput, single cell expression measurements that enable discovery of gene expression dynamics and molecular profiling of individual cell types. Cell surface protein information can be added with the use of Totalseq Antibodies.

The Chromium Single Cell V(D)J Solution enables profiling of full-length paired V(D)J transcripts from thousands of lymphocytes.  This solution allows assembly of full-length V(D)J sequences on a cell-by-cell basis, providing high-resolution insights into the adaptive immune system. Gene expression can be combined with immunophenotyping using the Totalseq Antibodies to better describe immune cell subsets and functional states.

The Assay for Transposase Accessible Chromatin (ATAC) for epigenomic analysis of thousands of individual nuclei provides insight into cell types and cell states for deeper understanding of gene regulatory mechanisms.

Designed to simultaneously profile gene expression and open chromatin from the same cell allowing characterisation of cell types and states, and uncover gene regulatory programs.

A defined set of transcripts from single cells or tissue sections can be profiled with Targeted Gene Expression. Customizable, comprehensive gene panels will allow reduced sequencing costs of your samples.

Available panels include Human Pan-Cancer Panel, Human Immunology Panel, Human Gene Signature Panel and the Human Neuroscience Panel. It is also possible to design custom panels and further information can be found on the 10X site.

This technology allows mapping of the whole transcriptome with morphological context of the tissue. Oxford Genomic Centre can support library preparation and sequencing of cDNA samples derived from Visium solution.

• Antibodies for cell surface protein analysis can be added for an extra layer of information. This solution is compatible with 3 prime and V(D)J Solutions. Individual antibodies or pre-designed panels are available from Biolegend.
• 10X’s CellPlex solution for cell hashing and multiplexing uses a series of oligonucleotides conjugated to a lipid, allowing for subsequent pooling of multiple samples to be run on single channel. Benefits of hashing include a reduction of doublet rate, minimising of batch effects and a cost reduction of complex experiment
• Peptide-MHC multimers to measure antigen specificity. Assess the specificity of the adaptive immune system at single cell resolution with simultaneous capture of paired T cell receptors and peptide-MHC multimers, or immunoglobulin heavy and light chains and their cognate antigens.